Zone Electrophoresis of Enzymes in Bacterial Taxonomy

Zone electrophoresis of enzymes in bacterial taxonomy.

The electrophoretic mobilities in starch gels have been determined for eight enzymes in extracts of representative cultures of members of the family Enterobacteriaceae. These values were compared with each other and with those obtained from certain bacteria not in this family. The migrations of the eight enzymes were virtually identical for each of eight strains of Escherichia coli and for two ...

Capillary zone electrophoresis pdf

Zone Behavior of Enzymes

1. The kinetics of the reversible combination of one enzyme center with one molecule of a substrate or inhibitor is treated as a true bimolecular instead of a pseudomonomolecular reaction. The general equations describing such a reaction are presented and analyzed algebraically and graphically. 2. A new term, "specific concentration," is introduced to denote the concentration of reactants in un...

Separation of enzymes from microorganism crude extracts by free-flow zone electrophoresis.

Continuous, single-step, state-of-the-art preparative separations of enzymes from microorganism crude extracts by free-flow zone electrophoresis are presented. In the first example, the enzymes formate dehydrogenase, formaldehyde dehydrogenase, and methanol oxidase were continuously separated from Candida boidinii crude extract. Yields of 85% to 95% and purification factors between 3 and 7 were...

Electrophoresis of bacterial variants.